Istiklol had qualified to the play-off stage after stunning PFC Lokomotiv 1-0 in Tashkent in the Preliminary Round 2, while Al Ahli qualified as fourth placed in the 2018-19 Saudi Pro League.

Goalkeeper Mahdi Rahmati was the hero for Shahr Khodro, producing a brilliant shootout save to deny countryman Karim Ansarifard before Saeid Sadeghi struck the decisive blow after more than 120 minutes of hard-fought action.

Shahr Khodro’s success means there will be four Iranian clubs in the group stage, while Al Sailiya will need to wait at least a year longer for their maiden taste of Continental group stage football.

Esteghlal had arrived to the play-off stage on the back of a 3-0 win over Kuwait SC in the second preliminary round, while Al Rayyan were kicking-off their campaign at this stage on home soil after finishing fourth in the 2018-19 Qatar Stars League season.

Bunyodkor had reached the play-off stage after defeating Iraq’s Al Zawraa 4-1 in the Preliminary Round 2 a week earlier. Meanwhile, Al Ain were placed directly in this stage, having finished fourth in the 2018-19 UAE Pro League.

Andrew Nabbout’s second-half goal and a stellar performance from goalkeeper Lawrence Thomas proved the difference as Melbourne joined China PR’s Beijing FC, Chiangrai United from Thailand and Korea Republic heavyweights FC Seoul in Group E of Asia’s premier club competition.

By way of contrast, the result signalled a premature end to Kashima’s latest Continental adventure, with the 2018 title winners left to rue what could have been at a rain-sodden Kashima Soccer Stadium.

The 2013 runners-up will go into Group E when the tournament begins next month, where they will face Chinese Super League side Beijing FC, Thai champions Chiangrai United and Melbourne Victory from Australia.

Second half goals from Sei Muroya and Adaílton Da Silva stamped Tokyo’s ticket to the tournament proper, in the process ending Ceres Negros’ bid to become the first club from the Philippines to reach the group stage.

Tokyo’s win means the 2019 J1 League runners-up will join Ulsan Hyundai, Shanghai Shenhua and Perth Glory in Group F, which begins on February 11.